Prevalence of isolated bacterial and antibiotic resistant pattern of them in positive blood cultures isolated from patients admitted to different parts of Toohid Hospital of Sanandaj city (2013-2014)

Document Type : Original article

Authors

1 1. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran 3. Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran

2 2. Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran 3. Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran

Abstract

Introduction and Objective: The presence of live bacteria in the blood reflects blood infection, which if not appropriately treated, associated with high mortality. The aim of this study was to evaluate frequency rate of bacterial agents that caused blood infections and also antibiotic resistance pattern of them in patients admitted to Toohid Hospital in Sanandaj city during 2013-2014 years.
Materials and methods: Present study was performed on 3242 patients. Positive blood culture samples of patients were evaluated and after bacteria isolated, antibiotic sensitivity test with disk diffusion method according to Clinical Laboratory Standard Institute (CLSI) were on performed. Data were analyzed using SPSS 16 software and frequency rate determination.
Results: 180 positive blood culture samples were isolated during one year. The highest frequency rate was related to Staphylococcus epidermidis (51.11%) and lowest was related to Serratia (1.11%). The highest resistance was to vancomycin, amikacin, ampicillin, sulfametoxazol, cefotaxime, ceftizoxime, penicillin and tetracycline. The highest and lowest bacteria isolates were isolated in children (50 isolates) and emergency (5 isolates) and outpatient parts (5 isolates), respectively. The highest resistance in different parts of hospital was to ampicillin.
Conclusion: with appropriate antibiotics prescription, according to exact results of antibiogram and with sanitation in various parts of hospital, especially the children's part, can be proceed accurate treatment and reduce health costs.

Keywords


1.Archibald L, Phillips L, Monnet D, McGowan JE Jr, Tenover F, Gaynes R. Antimicrobial resistance in isolates from inpatients and outpatients in the United States: increasing importance of the intensive care unit. Clinical Infectious Diseases 1997; 24(2):211-5.
2.  Vaez H , Khosravi S,  Soleyman E. Antibiotic resistance pattern of common etiological agents of bloodstream infections isolated from patients Iran. Journal of Medical Microbiology 2012; 5(4): 52-58. [in Persian]
3. Cleven BEE, Palka-Santini M, Gielen J, Meembor S, Kro¨nke M, Krut O.  Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray.  Journal of Clinical Microbiology 2006; 44(7): 2389–97
4. Kang CI, Kim SH, Park WB, Lee KD, Kim HB, Kim EC, et al. Bloodstream infections caused by antibioticresistant gram-negative bacilli: risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome. Antimicrobial Agents and Chemotherapy 2005; 49(2):760-6.
5. Tenover FC. Mechanisms of antimicrobial resistance in bacteria. American Journal of Infection Control 2006; 34 (5 Suppl 1): S3-10.
6. Hsueh PR, Chen ML, Sun CC, Chen WH, Pan HJ, Yang LS, et al. Antimicrobial drug resistance in pathogens causing nosocomial infections at a university hospital in Taiwan, 1981–1999. Emerging Infectious Diseases 2002; 8(1): 63-8.
7. Mohammadimehr  M, Feizabadi MM, Bahadori A. Antibiotic resistance pattern of Gram negative Bacilli Caused nosocomial infections in ICUs in khanevadeh and golestan hospital in Tehran -2007. Annals of Military and Health Sciences Research 2011; 8 (4): 283-90. [in Persian]
8. Cockerill FR, Patel JB, Alder J, Bradford PA, Dudley MN, Eliopoulos GM, et al. Performance standards for antimicrobial susceptibility testing; twenty-third informational supplement, M100-S23. Clinical and Laboratory Standards Institute (CLSI) 2013; 33(1): 130-6.
9. Amiri P, Pournajaf A, Shavalipour A, Tayebi Z, Goudarzi H, Eslami G. Evaluation of antimicrobial resistance in the beta-lactamase producing Escherichia coli isolated from urinary tract infection in the patients referring to Taleghani hospital of Tehran. Tabari Journal of Preventive Medicine 2015; 1(2): 1121. [in Persian]
10. Bakhsi khaniki GH , Asgharisana F, Gaibi SH. Study of the role of common bacterial etiology in neonatal sepsis in Urumiah Shahid. New Cellular and Molecular Biotechnology Journal 2011; 1(3): 17-21. [in Persian]
 11. Tabatabaei ST. Frequency and antimicrobial susceptibility of bacteria isolated from urine, stool, and blood cultures of Rafsanjan University of Medical Sciences laboratories during 2003. Journal of Rafsanjan University of Medical Sciences 2008; 7(2): 105-112. [in Persian]
12. Zaragoza R, Artero A, Camarena JJ, Sancho S, González R, Nogueira JM. The influence of inadequate empirical antimicrobial treatment on patients with bloodstream infections in an intensive care unit. Clinical Microbiology and Infection 2003; 9(5):412-8.
13. Maleki A, SH Ebrahimian, M Omranii, A Ranjbar , A Mikaeili. Evaluation of blood culture of neonatas suspected septicaemia in Hazrate Masoomeh hospital of Kermanshah, Iran (2006). Medical Laboratory Journal 2009; 3(1): 45-51. [in Persian]
14. Didgar F, Sarmadian H, Ghasemikhah R. Antimicrobial resistance pattern of Gram –negative bacilli isolated of Vali-Asr Hospital wards in Arak. Iranian South Medical Journal 2014; 17(5): 938-47. [in Persian]
15. Babamahmoodi F, Ahangarkani F, Davoudi A. Hospital-acquired infections, bacterial causative agents and antibiotic resistance pattern in intensive care units at teaching hospitals in north of Iran. International Journal of Medical Investigation 2015; 4 (1); 152-60. 
16. Zarei M, Erami M, Kosha H, Mohammadi. A survey of antibiotic resistance pattern of isolated Staphylococcus coagulase negative species from patients with bacteremia in Shahid Beheshti hospital of Kashan. Iranian Journal of Medical Sciences 2012; 37 (3 Supplement 1): 251