The effect of the mutation in rs1255190244 polymorphism in APC gene on the incidence of colorectal cancer by Tetra ARMS-PCR method

Document Type : Original article

Authors

1 Master of Cellular and Molecular Biology, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

2 Assistant Professor of Cellular and Molecular Biology, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

3 Assistant Professor of Biochemistry, Institute of Law Enforcement Equipment and Technologies, NAJA Institute of Law Enforcement Sciences and Social Studies, Tehran, Iran

Abstract

Background and Aims: Mutations in the Adenomatous Polyposis Coli gene can cause colon cancer. Colorectal cancer is the growth of cancer cells in the colon or rectum. The aim of this study was to analyze mutations in the rs1255190244 gene point in the APC gene in patients with colorectal cancer in Tehran province.
Materials and Methods: From 2019 to 2020, 100 samples of 6-10 ml per patient were collected from patients with colorectal cancer who were definitively diagnosed. Then DNA of each sample was extracted and after checking the quality of the DNA by electrophoresis, the required primers were designed. In the next step, the amount of rs1255190244 polymorphism in the APC gene was investigated by Tetra ARMS PCR method. Finally, the results of the analysis and significance level (P <0.003) were considered with SPSS software.
Results: The results of this study show that the incidence of colorectal cancer at the rs1255190244 gene point in the patient group compared with the control group with P0.003> is significantly associated with the incidence of colon cancer.
Conclusion: From the results of this study, it can be concluded that rs1255190244 polymorphism can be used as a marker to prevent colon cancer, but further research is needed to investigate several other formations.

Keywords

References

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Navid No
Volume 25, Issue 82
September 2022
Pages 50-60

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