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Evaluation of the effect of antioxidant resveratrol on sperm biochemical matches and expression of caspase 3 and HSP70 genes in infertile stenotratospermia under freezing conditions

Document Type : Original article

Authors
Narges Ashoori 1
Shahrebanoo Orian 2
Akram Eidi 1
Kambiz Roshanaei 3
1 Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Biology, Faculty of Basic Sciences, Kharazmi University, Tehran, Iran
3 Department of Biology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran
10.22038/nnj.2022.61923.1325
Abstract
Introduction: One of the most important measures to increase sperm quality and consequently the success of assisted reproductive technology (ART) is to protect sperm against oxidative reactions during freezing. Zoratrol is an antioxidant compound that protects the body against chronic diseases by scavenging free radicals. The aim of this study was to evaluate the effect of resveratrol on human sperm functional parameters and to evaluate apoptosis and expression of HSP70 and Caspase3 genes after cryopreservation.
Materials and Methods: In this study, 40 men referred to the ACECR Infertility Center in Qom, all of whom met the inclusion criteria, were used as the target population. The target population was divided into two groups of men with normal WHO criteria and a second group of patients with asthenotracheal sperm. After sperm sampling, the sperm was frozen with different concentrations of resveratrol. Semen samples were examined for sperm parameters including concentration, morphology, motility, viability, reactive oxygen species (ROS), apoptosis and expression of HSP70 and caspase 3 genes.
 Results: Resveratrol significantly increased sperm motility by 0.02 and 0.05 mM (P≤0.001), but decreased compared to the control group with resveratrol by 0.25 and 0.1 mM (P≤0.001). With the exception of resveratrol 0.25 mM, all doses of resveratrol, especially 0.05 mM, increased sperm count (P˂0.05). All doses of resveratrol, especially 0.02 mM and 0.05 mM, reduced apoptosis and expression of HSP70 and caspase3 genes.
Conclusion: In general, resveratrol supplementation before cryopreservation significantly increased sperm quality and reduced the side effects of cryopreservation shock.
 
Keywords
Sperm
Resveratrol
Stenotratospermia
HSP70
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Navid No
Volume 25, Issue 81
Spring 2022
Pages 67-85