Investigation of antibacterial properties in Pistacia vera L extract on Staphylococcus aureus

Document Type : Original article

Authors

1 Associate Professor, Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, IRAN

2 MSc,, Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, IRAN

Abstract

Introduction: Secondary metabolites of plants such as polyphenolic compounds have numerous therapeutic properties. Herbal medicines contain phytochemical compounds with antimicrobial properties have a great importance in the treatment of various microbial infections. The aim of this study was the usage of Pistacia vera L skin (more than 400,000 tons per year) in extracting compounds with antibacterial properties.
Material and methods: P. vera cv. Kallehghuchi was obtained from Sirjan city in Iran and pistachio skin was extracted with four solvents (70% acetone, 50% ethanol, 50% methanol and water), then, the antimicrobial properties of the extracts were investigated on Staphylococcus aureus. For this purpose, the disc diffusion method was used.
Results: MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values were measured to determine the minimum bactericidal concentration and minimum inhibitory concentration of the extracts.  Acetone solvent had the highest antibacterial properties, with MIC and MBC values of 88.55 μg / ml and 354.16, respectively.
Conclusion: In fact, the presence of some bioactive compounds such as phenols and flavonoids in Pistacia vera L skin, with antimicrobial properties can have many applications in various industries such as food and pharmaceutical industries.Therefore, these important compounds can be obtained from wastes such as Pistacia vera L skin, which are produced in large quantities annually in the country, and can contribute to the economy of society.

Keywords


  1. Hanachi P, Salehizadeh S, Ramezani R, Zarringhalami R. Comparison of antioxidant and anti-bacterial activities of Ocimum basilicum and Impatiens walleriana and their anticancer properties on SKOV-3 cancer cell line. Food Science and Technology. 2020.10;17(106):95-107.
  2. Mokhtarpour A, Naserian AA, Valizadeh R, Mesgaran MD, Pourmollae F. Extraction of phenolic compounds and tannins from pistachio by-products. Annual Research & Review in Biology. 2014. 8:1330-8.
  3. Hanachi P, Zarringhalami R, Kaya E. Total Phenolic and Flavonoid Content and Antibacterial Properties of Polygonatum orientale Desf and Tiliadasystyla. Hormozgan Medical Journal. 2020.2;25(1):19-23.
  4. Zarringhalami R, Hanachi P, Ramezani Tamijani R. Cytotoxic effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 cancer cell lines. Iranian Journal of Pharmaceutical Sciences. 2020.16(4):9-16
  5. Hosseinzadeh H, Tabassi SA, Moghadam NM, Rashedinia M, Mehri S. Antioxidant activity of Pistacia vera fruits, leaves and gum extracts. Iranian journal of pharmaceutical research: IJPR. 2012;11(3):879.
  6. Parfitt D, Kallsen C, Maranto J. Pistachio cultivars. Center for Fruit and Nut Crop Research and Information, Pomology Dept., Univ. Calif., Davis CA. 1995:43-6.
  7. Roosta, H. R., and A. R. Sajjadinia. "Using an Infrared Gas Analyzer for Evaluation of Photosynthetic Activity in Some Iranian Pistachio Cultivars. Journal of Nuts . 2013: 47-51.
  8. Oliveira I, Sousa A, Ferreira IC, Bento A, Estevinho L, Pereira JA. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food and chemical toxicology. 2008. 1;46(7):2326-31.
  9. Nadernejad, Nazi, Ali Ahmadimoghadam, Javad Hossyinifard, and Shahram Poorseyedi. "Study of the rootstock and cultivar effect in PAL activity, production of phenolic and flavonoid compounds on flower, leaf and fruit in Pistachio (Pistacia vera L.)." Iranian Journal of Plant Biology. 2013. 95-109.
  10. Chakraborty M, Mitra A. The antioxidant and antimicrobial properties of the methanolic extract from Cocos nucifera mesocarp. Food Chemistry. 2008. 1;107(3):994-9.
  11. Mazzola PG, Jozala AF, Novaes LC, Moriel P, Penna TC. Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents. Brazilian Journal of Pharmaceutical Sciences. 2009. 45(2):241-8.
  12. Khoddami A, Wilkes MA, Roberts TH. Techniques for analysis of plant phenolic compounds. Molecules. 2013. 18(2):2328-75.
  13. Albu S, Joyce E, Paniwnyk L, Lorimer JP, Mason TJ. Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry. Ultrasonics sonochemistry. 2004. 1;11(3-4):261-5.
  14. Hanachi P, Zarringhalami R, Tamijani RR. Investigation of Antioxidant Properties of Polygonatum orientale Desf and Tilia dasystyla Extracts by Different Methods and Solvents. Hormozgan Medical Journal. 2018. 31;22(4).
  15. Azadedel, S., Hanachi, P., Saboora, A. Investigation on antioxidant activity of pistachio (Pistacia vera L.) skin extraction. Journal of Plant Research (Iranian Journal of Biology), 2018; 30(4): 722-731.
  16. Azade del S, Hanachi P, Saboora O. Investigation of soluble and insoluble tannins and anthocyanins assay in two Cultivar pistachio (Pistacia vera L). FSCT. 2016; 14 (63) :179-186
  17. Fernández-Agulló A, Pereira E, Freire MS, Valentao P, Andrade PB, González-Álvarez J, Pereira JA. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial crops and products. 2013. 1;42:126-32.
  18. Rajaei A, Barzegar M, Mobarez AM, Sahari MA, Esfahani ZH. Antioxidant, anti-microbial and antimutagenicity activities of pistachio (Pistachia vera) green hull extract. Food and chemical toxicology. 2010. 1;48(1):107-12.
  19. Oliveira I, Sousa A, Morais JS, Ferreira IC, Bento A, Estevinho L, Pereira JA. Chemical composition, and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food and Chemical Toxicology. 2008. 1;46(5):1801-7.
  20. Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totté J, Pieters L, Vlietinck AJ. Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. Journal of ethnopharmacology. 2002. 1;79(2):213-20.
  21. Rajaei, A., M. Barzegar, and Ma Saharian. Investigation on antioxidative and antimicrobial activities of pistachio (Pistachia vera) green hull extracts. Iranian Journal Of Food Science And Technology.2010: 111-120.