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Comparison of Antimicrobial Effects of Melissa Officinalis and Impatiens Walleriana Extracts against Several Species of Pathogenic Bacteria

Document Type : Original article

Authors
Parichehr Hanachi 1
Roya Sehat 2
Ameneh Elikaei 3
Roshanak Zarringhalami 2
1 Associate Professor, Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2 MSc, Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
3 Assistant Professor, Department of Biochemistry, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
10.22038/nnj.2021.53808.1249
Abstract
Introduction: Emergence of resistance to antimicrobial medications has led to the use of medicinal herbs for the treatment of infections. In this regard, this study aimed to investigate the antimicrobial effects of three extracts (i.e., ethanol/methanol, water, and acetone) of Melissa officinalis and Impatiens walleriana on four species of bacteria.
Materials and Methods: The selected bacteria, including Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Enterococcus were first activated in the culture medium of broth neutrinos. Subsequently, a suspension equivalent to half McFarland was prepared from the activated bacteria. Antimicrobial activities of the extracts were evaluated by microdilution broth and disc diffusion methods to determine the minimum inhibitory and bactericidal concentration values.
Results: Based on the results, the Impatiens walleriana acetone extract had the most antimicrobial effects on all four species of bacteria. Moreover, acetone and ethanol/methanol extracts of Impatiens walleriana had more antimicrobial effects on E. coli than ethanol/methanol and water extracts of Melissa officinalis. In case of S. aureus, the water and ethanol/methanol extracts of both plants had almost the same minimum bactericidal and inhibitory concentration values. Finally, it can be said that all three Melissa officinalis extracts had better antimicrobial effects on Enterococcus and B. subtilis than the three Impatiens walleriana extracts.
Conclusion: Among all extracts, acetone extract of Impatiens walleriana showed the highest antibacterial property. Given the results and the increasing resistance of bacteria to antibiotics, it is suggested to use antibacterial compounds of plants for the treatment of infectious diseases; nevertheless, further studies should be carried out in this regard.
Keywords
Disc diffusion
Impatiens walleriana
Melissa officinalis
Minimum inhibitory concentration
Minimum bactericidal concentration
  1. Neu HC. The crisis in antibiotic resistance. Science. 1992; 257(5073):1064-73.
  2. Cseke LJ, Kirakosyan A, Kaufman PB, Warber S, Duke JA, Brielmann HL. Natural products from plants. Florida: CRC Press; 2016.
  3. Brill S, Dean E. Identifying and harvesting edible and medicinal plants in wild (and not so wild) places. New York: Hearst Books; 1994.
  4. Sharopov FS, Wink M, Khalifaev DR, Zhang H, Dosoky NS, Setzer WN. Composition and bioactivity of the essential oil of Melissa officinalis L. growing wild in Tajikistan. Int J Trad Natl Med. 2013; 2(2):86-96.
  5. Fairbrother JM, Nadeau É, Desautels C. Non-pathogenic F18 E. coli strain and use thereof. United States patent US 10,258,654. Washington, DC: U.S. Patent and Trademark Office; 2019.
  6. Jackson KA, Gokhale RH, Nadle J, Ray SM, Dumyati G, Schaffner W, et al. Public health importance of invasive methicillin-sensitive Staphylococcus aureus infections: surveillance in 8 US counties, 2016. Clin Infect Dis. 2020; 70(6):1021-8.
  7. Zhang C, Huang J, Zhang J, Liu S, Cui M, An B, et al. Engineered Bacillus subtilis biofilms as living glues. Mater Today. 2019; 28:40-8.
  8. García-Solache M, Rice LB. The Enterococcus: a model of adaptability to its environment. Clin Microbiol Rev. 2019; 32(2):e000058.
  9. Chakraborty M, Mitra A. The antioxidant and antimicrobial properties of the methanolic extract from Cocos nucifera mesocarp. Food Chem. 2008; 107(3):994-9.
  10. Hanachi P, Salehizadeh S, Ramezani R, Zarringhalami R. Comparison of antioxidant and anti-bacterial activities of Ocimum basilicum and impatiens walleriana and their anticancer properties on SKOV-3 cancer cell line. Food Sci Technol. 2020; 17(106):95-107.
  11. Mazzola PG, Jozala AF, Novaes LC, Moriel P,
    Penna TC. Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents. Braz J Pharm Sci. 2009; 45(2):241-8.
  12. Rabbani M, Etemadifar Z, Karamifard F, Borhani MS. Assessment of the antimicrobial activity of Melissa officinalis and Lawsonia inermis extracts against some bacterial pathogens. Compar Clin Pathol. 2016; 25(1):59-65.
  13. Ehsani A, Alizadeh O, Hashemi M, Afshari A, Aminzare M. Phytochemical, antioxidant and antibacterial properties of Melissa officinalis and Dracocephalum moldavica essential oils. Vet Res Forum. 2017; 8(3):223-9.
  14. Abdellatif F, Boudjella H, Zitouni A, Hassani A. Chemical composition and antimicrobial activity of the essential oil from leaves of Algerian Melissa officinalis L. EXCLI J. 2014; 13:772-81.
  15. Miraj S, Rafieian-Kopaei, Kiani S. Melissa officinalis L: a review study with an antioxidant prospective. J Evid Based Complementary Altern Med. 2017; 22(3):385-94.
  16. Schnitzler P, Schuhmacher A, Astani A, Reichling J. Melissa officinalis oil affects infectivity of enveloped herpesviruses. Phytomedicine. 2008; 15(9):734-40.
  17. Haider F, Ullah N. Antioxidant and antimicrobial activity of impatiens walleriana local to Malaysia. Moroccan J Chem. 2019; 7(3):7-3.
  18. Delgado-Rodriguez FV, Hidalgo O, Loría-Gutiérrez A, Weng-Huang NT. In vitro antioxidant and antimicrobial activities of ethanolic extracts from whole plants of three Impatiens species (balsaminaceae). Ancient Sci Life. 2017; 37(1):16.

 

Navid No
Volume 24, Issue 77
Spring 2021
Pages 41-49