Evaluation of Antifungal Effects of Prangos ferulace and Plantago major L Plants Against Fluconazole-resistant Candida albicans Species in Extracorporeal Conditions

Document Type : Original article

Authors

1 PhD in Biochemistry, Cellular and Molecular Research Center of Yasuj University of Medical Sciences, Iran

2 PhD Student of Medical Biotechnology, Student Research Committee, Faculty of Paramedicine, Iran University of Medical Sciences, Tehran, Iran

3 Msc in Microbiology, Department of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran

4 Msc in Agriculture, Yasuj University, Yasuj, Iran

Abstract

Introduction: Medicinal plants can be effective in treating diseases due to having various antifungal compounds. The aim of this study was to evaluate the antifungal effects of Prangos ferulace and Plantago majorL against fluconazole-resistant Candida albicans in extracorporeal conditions.
Materials and Methods: This study was experimental. Prangos ferulace and Plantago majorL plants were prepared from stores in Yasuj, Iran, in the spring and then scientifically approved by the Zardband Pharmaceutical Company in Yasuj. The extract was extracted after sending the plants to the Research Center for Medicinal Plants of Yasuj University of Medical Sciences. In order to prepare the aqueous extract of the plants, 3 g of plant powder was added to 30 ml of distilled water and placed on a shaker (Behdad, Tehran, Iran) for 24 h. The strain used in the present study was Candida albicans ATCC 10231, which was prepared by Shiraz University of Medical Sciences, Shiraz, Iran. In the present study, the alcoholic extracts of Prangos ferulace and Plantago major L were used at the concentrations of 7.5, 15, and 30 mg/ml. The non-growth halo diameter (in millimeters) was measured using the standard paper disc diffusion method. The minimum inhibitory concentrations (MICs) required to inhibit the growth of 90% and 50% of organisms were also evaluated. Finally, the number of living cells was counted, and the data were analyzed using ANOVA test in SPSS software (version 23).
Results: The results showed that at a concentration of 30 mg/ml, the maximum diameters of the non-growth halo were 15.3 mm for Prangos ferulace and 12.4 mm for Plantago major L. Regarding the MIC value, the MIC50 and MIC90 were respectively obtained as 19 and 38 μg/ml for Prangos ferulace and 14 and 29 μg/ml for Plantago major L (p < 0.05). Considering the live cell count, the lowest number of live cells was formed at a dilution of 10. The mean value of this variable was obtained as 7.3 and 4.2 CFU/ml for Prangos ferulace and Plantago major, respectively.
Conclusion: Prangos ferulace and Plantago major L. prevented the activity of Candida albicans due to their effective medicinal compounds.

Keywords

References

  1. Edalat Panah Yadolah, Enayati Parvar  Fariba, Rajaee Nejad Sajjad. Investigating the effect of local rangeland honey on Kohgiluyeh and Boyer-Ahmad Provinces on the activity of Candida albicans. J Navid No. 2020; 22(72):44-54.
  2. Vandenbosch D, Braeckmans K, Nelis HJ, Coenye T. Fungicidal activity of miconazole against Candida spp. Biofilms. J Antimicrob Chemother. 2009; 65(4):694-700.
  3. Al-Waili NS. Mixture of honey, beeswax and olive oil inhibits growth of Staphylococcus aureus and Candida albicans. Arch Med Res. 2005; 36(1):10-3.
  4. Khodavandi A, Tahzir NA, Cheng PW, Chen PY, Alizadeh F, Hrmal NS, et al. Antifungal activity of Rhizome coptidis and Alpinia galangal against Candida species. J Pure Appl Microbiol. 2013; 7:1725-30.
  5. Kruppa M. Quorum sensing and Candida albicans. Mycoses. 2009; 52(1):1-10.
  6. Amiri H. Chemical composition and antibacterial activity of essential oil of Prangos ferulacea (L.) Lindl. J Med Plants. 2007; 1(21):36-41.
  7. Guo Y, He Y, Guo N, Gao J, Ni Y. Variations of the composition of the leaf cuticular wax among Chinese populations of Plantago major. Chem Biodivers. 2015; 12(4):627-36.
  8. Khanjani N, Zamanian M, Molazadeh P, Sadeghi M. The prevalence of Candida albicans infection and related factors in women referring to health centers of Jiroft in 2010: a short report. J Rafsanjan Univ Med Sci. 2014; 13(6):569-76.
  9. Zia M, Bayat M, Khalkhali H. In vitro antifungal effect of Thymus vulgaris essence on Candida albicans isolated from patients with oral candidiasis. J Shahrekord Univ Med Sci. 2011; 13(3):44-52.
  10. Naeini A, Khosravi AR, Chitsaz M, Shokri H, Kamlnejad M. Anti-Candida albicans activity of some Iranian plants used in traditional medicine. J Mycol Med. 2009; 19(3):168-72.
  11. Banaean-Boroujeni S, Rasti-Boroujeni M, Moghim H, Validi M, Mobini G, Kazemian A. In vitro effect of honey on Candida albicans and lactobacillus. J Shahrekord Univ Med Sci. 2010; 11(4):52-8. [in Persian].
  12. Sayyah M, Moaied S, Kamalinejad M. Anticonvulsant activity of Heracleum persicum seed. J Ethnopharmacol. 2005; 98(1-2):209-11.
  13. Allen KL, Molan PC, Reid GM. A survey of the antibacterial activity of some New Zealand honey. J Pharm Pharmacol. 1991; 43(12):817-22.
  14. Shareyat SH. Qualitative and quantitative evaluation of the active constituents and control methods for medicinal plants. 1st ed. Isfahan: Mani Pub; 1998. P. 10-27.
  15. Omran M, Maliji GH, Sefidgar A, Yosefi MR, Haji Ahmadi M, Moosavi S, et al. effect of honey from north of Iran on Candida albicans. J Babol Univ Med Sci. 2008; 10(5):15-22.
  16. Nazemi Salman B, Yazdinejad A, Salah S, Sajedinejad N. Antifungal activity of essential oils of Prangos frulacea, Ziziphora tenuior, Ferula gummosa and Dracocephalum moldavica against Candida albicans. J Qazvin Univ Med Sci. 2017; 21(1):4-11.
  17. Mayer FL, Wilson D, Hube B. Candida albicans pathogenicity mechanisms. Virulence. 2013; 4(6):
    119-28.
  18. Melo AS, Bizerra FC, Freymuller E, Arthington-Skagges BA, Colombo AL. Biofilm production and evaluation of antifungal susceptibility amongst clinical Candida spp. isolates, including strains of the Candida parapsilosis complex. Med Mycol. 2011; 49(3):253-62.
  19. Movaghari Pour A, Sheikh Fathollahi M, Poor Zamani M, Abedini S, Jamali Z. Comparison of anti-fungal effect of origanum vulgare extract versus nystatin on Candida albicans; an in vitro study. J Mashhad Dent Sch. 2018; 42(3):277-1.
  20. Vale-Silva L, Silva MJ, Oliveir D, Goncalves M, Cavaleiro C, Salgueiro L, et al. Correlation of the chemical composition of essential oils from Origanum vulgare subspecies. virens with their in vitro activity against pathogenic yeasts and filamentous fungi. J Med Microbiol. 2012; 61(2):252-60.
  21. Aminian R, Mardani M, Davoodnia B. The effect of hydro alcoholic extract of Plantago major and Astragalus hamosus on some gram-positive and gram-negative bacteria. J Plant Res. 2018; 31(3): 956-67.
  22. Akbari S. Antifungal activity of thymus vulgaris L. and Origanum vulgare L. against fluconazol-resistant and susceptible Candida albicans isolates. J Med Plants. 2007; 6(Suppl 3):53-62.
  23. Kafash-Farkhad N, Asadi-Samani M, Khaledifar B. A review on secondary metabolites and pharmacological effects of Prangos ferulacea (L.) Lindl. J Shahrekord Univ Med Sci. 2013; 15(3):98-108.
Navid No
Volume 23, Issue 74
September 2020
Pages 44-52

Files

Share

How to cite

Statistics