Detection of Capsular Types in Klebsiella Pneumoniae Strains isolated from Bovine Mastitis

Document Type : Original article

Authors

1 MSc in Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Professor, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Introduction: Mastitis is one of the most common diseases in dairy cows which causes irreparable damages to the livestock industry in Iran and other regions worldwide. Klebsiella pneumonia based on K capsular antigen include serotypes, such as K1, K2, K5, K54, and K57. The aim of this study was to detect this bacterium in clinical and subclinical mastitis cases of cows and determine its common capsular serotypes.
Materials and Methods: In this study, milk samples were collected from 100 lactating cows with mastitis from a large dairy farm in Chaharmahal and Bakhtiari in the second half of 2017. Klebsiella pneumonia strains were isolated after microbial culture and molecular confirmation. The presence of K capsular antigens in these isolates was assessed using Polymerase Chain Reaction method.
Results: Out of 100 samples, 20 samples (20%) were found infected with Klebsiella pneumonia using microbial culture and 16S-23S ITS gene tracing. The frequency of capsular antigens K1, K2, K5, K54, and K57 were 0.8%, 2.4%, 0, 1.2%, and 0.4% respectively. Moreover,the capsular antigen K2 with a frequency of 2.4% and K5 with a frequency of 0% had the highest and lowest frequency, respectively. The data were analyzed using SPSS 21 AND EXCEL through the chi-square test with a 95% confidence interval.
Conclusion: According to the results, Klebsiella pneumonia bacteria had a low frequency in the samples of the present study. Therefore, it is hoped that the diagnosis and evaluation of these bacterial agents which lead to the effective development of mastitis control and prevent Klebsiella pneumonia by providing a national program, thereby reducing the costs of this disease.

Keywords

References

  1. Paczosa MK, Mecsas J. Klebsiella pneumoniae: going on the offense with a strong defense. Microbiol Mol Biol Rev. 2016; 80(3):629-61.
  2. Heidary M, Nasiri MJ, Dabiri H, Tarashi S. Prevalence of drug-resistant Klebsiella pneumoniae in Iran: a review article. Iran J Public Health. 2018; 47(3):317-26.
  3. Bengoechea JA, Sa Pessoa J. Klebsiella pneumoniae infection biology: living to counteract host defences. FEMS Microbiol Rev. 2019; 43(2):123-44.
  4. Navon-Venezia S, Kondratyeva K, Carattoli A. Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. FEMS Microbiol Rev. 2017; 41(3):252-75.
  5. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018; 8:4.
  6. Follador R, Heinz E, Wyres KL, Ellington MJ, Kowarik M, Holt KE, et al. The diversity of Klebsiella pneumoniae surface polysaccharides. Microb Genom. 2016; 2(8):e000073.
  7. Feizabadi MM, Raji N, Delfani S. Identification of Klebsiella pneumoniae K1 and K2 capsular types by PCR and quellung test. Jundishapur J Microbiol. 2013; 6(9):e7585.
  8. Mohammad SM, AskariBadouei M, Gorji DM, Daneshvar M, Koochakzadeh A. A study on the clinical Coliform mastitis of Holstein cows on Garmsar suburban dairy farms. Vet Res. 2012; 8(2):137-49. [in Persian]
  9. Schukken YH, Barkema HW, Lam TJ, Zadoks RN. Improving udder health on well managed farms: mitigating the perfect storm. International Conference on the Mastitis Control from Science to Practice. Haque. 2008; 10(2):21-35.
  10. Bradley AJ, Green MJ. A study of the incidence
    and significance of inframammary enter bacterial infections acquired during the dry period. J Dairy Sci. 2000; 83(9):1957-65.
  11. Liu Y, Liu C, Zheng W, Zhang X, Yu J, Gao Q, et al. PCR detection of Klebsiella pneumoniae in infant formula based on 16S-23S internal transcribed spacer. Int J Food Microbiol. 2008; 125(3):230-5.
  12. Tavakol M, Momtaz H. Molecular characterization of serotypes and capsular virulence genes in cps gen group of Klebsiella pneumonia isolated from Tehran hospitals. J Microbial World. 2017; 10(1):18-25. [in Persian]
  13. Munoz MA, Ahlström C, Rauch BJ, Zadoks RN. Fecal shedding of Klebsiella pneumoniae by dairy cows. J Dairy Sci. 2006; 89(9):3425-30.
  14. Firouzi R, Rajaian H, Tabaee IM, Saeedzadeh A. In vitro antibacterial effects of marbofloxacin on microorganisms causing mastitis in cows. J Vet Res. 2010, 65(1):51-5.
  15. Saleki K, Moradi H. Bacterial agents of mastitis in dairy cow farms in Ilam city. Sci J Ilam Univ Med Sci. 2013; 20(4):88-95. [in Persian]
  16. Sharififar M, Mohsenzadeh M, Fallahrad A. Health evaluation and infection rates of cows with oram minnie in a number of industrial dairy farms of Mashhad. Milk Health Sem. 2009; 12(22):1-2. [in Persian]
  17. Munoz MA, Welcome FL, Schukken YH, Zadoks RN. Molecular epidemiology of two Klebsiella pneumoniae mastitis outbreaks on a dairy farm in New York State. J Clin Microbiol. 2007; 45(12):3964-71.
Navid No
Volume 22, Issue 71
December 2019
Pages 11-18

Files

Share

How to cite

Statistics