Optimization of Lymphocyte Cell Culture for Karyotype Using a Platelet-rich Plasma Method

Document Type : Original article

Authors

1 MSc, Instructor, Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

2 PhD, Assistant Professor, Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

Abstract

Introduction: Karyotype is one of the most popular and most widely used tests in genetic laboratories throughout the country. In this study, the karyotype diagnostic method and platelet-rich plasma therapy (PRP) have been integrated into each other to improve the quality of the karyotype test. This technique is essential in genetic labs where blood samples are deficient, or few cells are available for a karyotype test.  The aim of this study was to optimize lymphocyte cell cultures for performing a karyotype test using a platelet-rich plasma method.
Materials and Methods: This applied study included 30 cases from Shahrekord University in 2013. Platelet-rich plasma therapy was used to increase white blood cell and decrease red blood cells as well as other annoying and unnecessary factors in cell culture media to improve the lymphocyte cell culture.
Results: The final slides of karyotypes made with this method had excellent quality and a reasonably transparent background, compared to the conventional methods. It should be noted that in the final slide, there were more chromosomes than those in the conventional karyotype method.
Conclusion: The better quality of the chromosomes in the karyotype slides makes the study of the chromosomes' structure very convenient and more accurate. On the other hand, the more chromosomes are retained in the karyotype slides which improve their analysis using special karyotype software and make the detection of structural mosaicism more accurately in karyotype slides.

Keywords

References

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Navid No
Volume 22, Issue 70
September 2019
Pages 45-58

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