Distribution of virulence factors in biofilm formation strains of Pseudomonas aeroginosa isolated from hospital infections in Shahrekord Township

Document Type : Original article

Authors

1 Professor of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

2 Post graduated of Microbiology, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 Post graduated of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Background and Aim: Pseudomonas aeroginosa is one of the widespread clinical-caused infections which has many stringency factors and causes different clinical infections in humans and animals. This study was performed to investigate the relationship between biofilm formation and virulence factors distribution in Pseudomonas aeroginosa strains isolated from clinical samples.
Materials and Methods: In this study, 33 isolates of Pseudomonas aeroginosa were isolated from different clinical infections from the patients hospitalized in hospitals and treatment centers of Shahrekord city in the first 6 months of 1397. Then the ability of biofilm formation was investigated with the microtiter plate method and with the presence of most commonly virulence factors included pudA, phzI/II/H/M, apr, picH/N, exoU/S/Y/T, toxA, lasA/B, algD/U and pilA/B genes in these isolates using multiplex PCR method.
Results: In this study, 30 isolates had the ability of formation of powerful biofilm. The types of genes were detected in them which among these genes exoU and exoT genes were recognized as commonly stringency factors with the affluence of %93.33 and %86.66 respectively.
Conclusion: The presence of some virulence genes especially exotoxin-caused genes, lasA/B and Pilli-caused genes in these isolates indicated the importance role of these factors in Pseudomonas aeroginosa in biofilm formation and colonizing on epithelial surfaces.

Keywords

References

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Navid No
Volume 21, Issue 67
December 2018
Pages 30-40

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