Prevalence of ebp A, ebp B and ebp C genes in Enterococcus faecalis producing biofilm isolated from meat in Shahrekord

Document Type : Original article

Authors

1 MSc in Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Associate Professor, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

3 Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Introduction: Enterococcus, as a bacterium resistant to most antibiotics, is capable of releasing resistant genes to other species. Due to its ability to form biofilms, it has high pathogenicity. The purpose of this study was to investigate the frequency of Enterococcus faecalis in beef meat sold in Shahrekord retail markets and to investigate the relationship between ebp-A, ebp-B and ebp-C genes in biofilm-producing Enterococcus faecalis isolated from meat.
Materials and Methods: In this descriptive cross-sectional study, 80 meat samples were examined by biochemical and molecular methods. Detection of Enterococcus faecalis ebp-A, ebp-B and ebp-C genes was performed in the presence of specific primers. Statistical analysis was performed using Prism7 software and Fisher’s exact test.
Results: Of the 80 samples, meat samples were detected based on biochemical responses in 31 isolates of Enterococcus faecalis. Using the micro titer plate method, in 25 isolates (80.64%) biofilms were produced. ebp-A gene in 28 isolates (32.90%), ebp-B gene in 10 isolates (25.23%) and ebp-C gene in none of the isolates (0%) were not reported.
Conclusion: Foodborne diseases are one of the common problems in public health. Bacteria are the most important causes of infections and food poisoning. To prevent microbial contamination of foods, training individuals, observing health and supervision in the preparation, transportation, storage and delivery of foods are essential.

Keywords

References

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Navid No
Volume 21, Issue 66 - Serial Number 66
September 2018
Pages 41-51

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