Genotyping of vacA and cagA in Helicobacter pylori Strains in Saliva and Feces of Isfahan’s Children

Document Type : Original article

Authors

1 Post Graduated of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Professor of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

3 Assistant Professor of Microbiology, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Introduction:Helicobacter pylori is a factor causingdigestive diseases. The studies have been done on this disease revealed that polymorphism of acute factors of this bacteria (vacA and cagA) can determine its ability in creating different types of digestive diseases. This research aimed to determine the genotypes of vacA and cagAin Helicobacter pylori strains in saliva and stool of Isfahan’s children. 
Materials and Methods:In this cross-sectional study, 52 sample, from March 2015 to July 2016, for were taken from saliva and stool of Isfahan’s children under 10 years old suffered from gastrointestinal disorders, especially gastric reflux. These children were positive of anti- Helicobacter pylori IgM in ELISA test. DNA was extracted from saliva and stool samples the alleles of vacA and cagA were determined by PCR.
Results:Following the PCR test on the studied samples, 7 samples of saliva (13.46%) a 1 sample of stool (1.92%) had ureC of Helicobacter pylori and all of the positive samples, had cagA. In addition, all of 8 positive samples to Helicobacter pylori had the genotype of s2/m2 from the viewpoint of vacA.
Conclusion:According to the results, genotype of s2/m2 from the viewpoint of vacAshould be considered as an index for prediction of severe digestive diseases in this region. To be sure, doing wider molecular studies in other populations is recommended.
 

Keywords

References

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Navid No
Volume 20, Issue 64
March 2018
Pages 24-33

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