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Study of vac A genotypes of H.pylori isolated from patients with the upper gastrointestinal diseases by PCR

Document Type : Original article

Authors
seyed asghar havaei 1
Rasool Salehi 2
seyed ali fazeli 1
hamid tavakoli 3
Parviz Mohajeri 4
1 Associate Professor of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences
2 Dept. of Cell Biology, Schcool of Medicin, Isfahan Unive of Med. Sciences
3 Schcool of Medicin, Isfahan Unive of Med. Sciences
4 School of Medicin, Kerman Shah , Unive of Med. Sciences
10.22038/nnj.2015.5624
Abstract
Background:
Helicobacter pylori is a gram negative curved bacilli which is colonized in the human stomach. It causes duodental ulcer, gastritis and is associated with adenocarinoma. There might be a possible relationship between cagA, vacA and ice a genes and clinical outcomes. The aim of this study was to identify the frequency od vacA genotypes of H.pylori isolated from the upper gastrointestinal.
Methods:
100 H. pylori strains were isolated from patients with different gastrointestinal disease in Azahra hospital. Vac A alleles were typed using PCR with specific primers.
Finding :
There were four Vac A mosaicsms, including 28 for s1a/ m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%), s2 form was not found.
Conclusion:
The results showed there is no significant relationship between fifferent genotypes of vacA and the related diseases.
Keywords
Helicobacter pylori- vac A- Adenocarinoma
Duodental ulcer- Normal /Gastritis
PCR