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Detection of Aquatic Streptomyces with Antimicrobial Effect on Gentamycin-resistant Escherichia coli in Bakhtegan Lake in Fars, Iran

Document Type : Original article

Authors
Mohammad Masoud Eskandari 1
Behin Omidi 2
Mehdi Dehghani Zahedi 3
1 MSc in Microbial Biotechnology, Islamic Azad University, Central Tehran Branch, Tehran, Iran
2 Assistant Professor, Department of Biology, Islamic Azad University, Central Tehran Branch, Tehran, Iran
3 Assistant Professor, Department of Biology, Islamic Azad University, Yazd Branch, Yazd, Iran
10.22038/nnj.2020.45245.1192
Abstract
Introduction: Following the resistance of bacteria to antibiotics, the prevalence of antibiotic-resistant Escherichia coli (E. coli)strains has become a major medical challenge in recent years. It demands the development of effective antibiotics with natural origin. Actinomycetes are known for their ability to produce secondary metabolites and antibiotics. The aim of this study was to isolate aqueous Streptomyces species producing antimicrobial agents against gentamicin-resistant E. coli in Lake Bakhtegan in Fars province, Iran.
Materials and Methods: The first step was to obtain samples to evaluate the antagonistic effect of Streptomyces on antibiotic-resistant E. coli. In this study, 10 water samples were collected from Bakhtegan Lake. The bacteria were cultured in a specific culture medium (i.e., ISP2), and Streptomyces species were isolated and purified. The isolation and determination of the efficacy of secondary metabolites in the pathogens were performed by disk diffusion method. Deoxyribonucleic acid was extracted from effective Streptomyces for molecular analysis. The 16S ribosomal ribonucleic acid sequence was excised, and polymerase chain reaction was conducted using 27F and 1492R primers to identify the Streptomyces species.
Results: In this study, 17 Streptomycin isolates were recovered from 10 water samples 7 of which secreted metabolites. In addition, one isolate with a more strong effect on gentamicin-resistant E. coli was sequenced and identified as Streptomyces species strain 11K402.
Conclusion: Based on the obtained results of this study, it can be concluded that aquatic environments are considered suitable sources for the detection and isolation of Actinomycetes and Streptomycetes with antimicrobial metabolites.
 
Keywords
Drug resistance
Escherichia coli
Streptomycetes
  1. Lepuschitz S, Huhulescu S, Hyden P, Springer B, Rattei T, Allerberger F, et al. Characterization of a community-acquired-MRSA USA300 isolate from a river sample in Austria and whole genome sequence based comparison to a diverse collection of USA300 isolates. Sci Rep. 2018; 8(1):9467.
  2. Klein EY, Van Boeckel TP, Martinez EM, Pant S, Gandra S, Levin SA, et al. Global increase and geographic convergence in antibiotic consumption between 2000 and 2015. Proc Natl Acad Sci. 2018; 115(15):E3463-70.
  3. Oberlé K, Capdeville MJ, Berthe T, Budzinski H, Petit F. Evidence for a complex relationship between antibiotics and antibiotic-resistant Escherichia coli: from medical center patients to a receiving environment. Environ Sci Technol. 2012; 46(3):
    1859-68.
  4. Rochford C, Sridhar D, Woods N, Saleh Z, Hartenstein L, Ahlawat H, et al. Global governance
    of antimicrobial resistance. Lancet. 2018; 391
    (10134):1976-8.
  5. Mohammadipanah F, Wink J. Actinobacteria from arid and desert habitats: diversity and biological activity. Front Microbiol. 2016; 6:1541.
  6. Walsh CT, Wencewicz TA. Prospects for new antibiotics: a molecule-centered perspective. J Antibiot. 2014; 67(1):7-22.
  7. Thevenon F, Adatte T, Wildi W, Poté J. Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland). Chemosphere. 2012; 86(5):468-76.
  8. Genilloud O. Mining actinomycetes for novel antibiotics in the omics era: are we ready to exploit this new paradigm? Antibiotics. 2018; 7(4):E85.
  9. Bérdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot. 2012; 65(8):385-95.
  10. Singh LS, Baruah I, Bora TC. Actinomycetes of Loktak habitat: isolation and screening for antimicrobial activities. Biotechnology. 2006; 5(2):
    217-21.
  11. Saadoun I, Al-Joubori B, Al-Khoury R. Testing of production of inhibitory bioactive compounds by soil Streptomycetes as preliminary screening programs in UAE for anti-cancer and anti-bacterial drugs. Int J Curr Microbiol Appl Sci. 2015; 4(3):446-59.
  12. Cayol JL, Ollivier B, Alazard D, Amils R, Godfroy A, Piette F, et al. The extreme conditions of life on the planet and exobiology. Environ Microbiol. 2015; 2(10):353-94.
  13. Ahmad MS, El-Gendy AO, Ahmed RR, Hassan HM, El-Kabbany HM, Merdash AG. Exploring the antimicrobial and antitumor potentials of Streptomyces sp. AGM12-1 isolated from Egyptian soil. Front Microbiol. 2017; 8:438.
  14. Dilip CV, Mulaje SS, Mohalkar RY. A review on actinomycetes and their biotechnological application. Int J Pharm Sci Res. 2013; 4(5):1730.
  15. World Health Organization. Antimicrobial resistance and primary health care. Geneva: World Health Organization; 2018.
  16. Gurovic MV, Olivera NL. Antibacterial producing actinomycetes from Extra Andean Patagonia. J Arid Environ. 2017; 144:216-9.
  17. Barka EA, Vatsa P, Sanchez L, Gaveau-Vaillant N, Jacquard C, Klenk HP, et al. Taxonomy, physiology, and natural products of Actinobacteria. Microbiol Mol Biol Rev. 2016; 80(1):1-43.
  18. Salwan R, Sharma V. The role of actinobacteria in the production of industrial enzymes. New and future developments in microbial biotechnology
    and bioengineering. New York: Elsevier; 2018.
    P. 165-77.
  19. Landwehr W, Wolf C, Wink J. Actinobacteria and myxobacteria-two of the most important bacterial resources for novel antibiotics. How to overcome the antibiotic crisis. Basel, Switzerland: Springer; 2016. P. 273-302.
  20. Harwani D. Biodiversity of rare thermophilic actinomycetes in the great Indian Thar desert: an overview. Ind Am J Pharm Res. 2013; 3:9349-56.
  21. Tiwari K, Gupta RK. Rare actinomycetes: a potential storehouse for novel antibiotics. Crit Rev Biotechnol. 2012; 32(2):108-32.
  22. Nontongana N, Sibanda T, Ngwenya E, Okoh AI. Prevalence and antibiogram profiling of Escherichia coli pathotypes isolated from the Kat River and the Fort Beaufort abstraction water. Int J Environ Res Public Health. 2014; 11(8):8213-27.
  23. Dar OA, Hasan R, Schlundt J, Harbarth S, Caleo G, Dar FK, et al. Exploring the evidence base for national and regional policy interventions to combat resistance. Lancet. 2016; 387(10015):285-95.
  24. D’Costa VM, King CE, Kalan L, Morar M, Sung WW, Schwarz C, et al. Antibiotic resistance is ancient. Nature. 2011; 477(7365):457-61.
  25. Bhullar K, Waglechner N, Pawlowski A, Koteva K, Banks ED, Johnston MD, et al. Antibiotic resistance is prevalent in an isolated cave microbiome. PloS One. 2012; 7(4):e34953.
  26. Yin Q, Yue D, Peng Y, Liu Y, Xiao L. Occurrence and distribution of antibiotic-resistant bacteria and transfer of resistance genes in Lake Taihu. Microbes Environ. 2013; 28(4):479-86.
  27. Ser HL, Palanisamy UD, Yin WF, Abd Malek SN, Chan KG, Goh BH, et al. Presence of antioxidative agent, Pyrrolo [1, 2-a] pyrazine-1, 4-dione, hexahydro-in newly isolated Streptomyces mangrovisoli sp. nov. Front Microbiol. 2015; 6:854.
  28. Baquero F, Martínez JL, Cantón R. Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol. 2008; 19(3):260-5.
  29. Pourmand A, Mazer-Amirshahi M, Jasani G, May L. Emerging trends in antibiotic resistance: Implications for emergency medicine. Am J Emerg Med. 2017; 35(8):1172-6.
  30. Lyimo B, Buza J, Subbiah M, Smith W, Call DR. Comparison of antibiotic resistant Escherichia coli obtained from drinking water sources in northern Tanzania: a cross-sectional study. BMC Microbiol. 2016; 16(1):254.
  31. Li WJ, Zhang YG, Zhang YQ, Tang SK, Xu P, Xu LH, et al. Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete. Int J Syst Evol Microbiol. 2005; 55(3):1329-33.
  32. Gebreyohannes G, Moges F, Sahile S, Raja N. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Biomed. 2013; 3(6):426-35.
  33. Singh LS, Sharma H, Talukdar NC. Production
    of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak Lake of Manipur, India. BMC Microbiol. 2014; 14(1):278.
  34. Kafilzadeh F. Dehdari F. Namdar N. Isolation and evaluatin of marine actinomycetes from mangrove forests in south of Iran against some human bacterial pathogens. J Ardabil Univ Med Sci. 2013; 13(1):69-77 [in Persian].
  35. Darabpour E, Ardakani MR, Motamedi H, Ronagh MT. Isolation of apotent antibiotic producer bacterium, especially against MRSA, from northern region of the Persian Gulf. Bosnian J Basic Med Sci. 2012; 12(2):108 [in Persian].
  36. Undabarrena A, Beltrametti F, Claverías FP, González M, Moore ER, Seeger M, et al. Exploring the diversity and antimicrobial potential of marine actinobacteria from the comau fjord in Northern Patagonia, Chile. Front Microbiol. 2016; 7:1135.
  37. Tang SK, Li WJ, Dong W, Zhang YG, Xu LH, Jiang CL. Studies of the biological characteristics of some halophilic and halotolerant actinomycetes isolated from saline and alkaline soils. Actinomycetologica. 2003; 17(1):6-10.
Navid No
Volume 23, Issue 73
Spring 2020
Pages 22-34